| 벤자틴 페니실린에 의해 유도된 국소 발작간 간질양 파형이 백서 대뇌에서 c-JUN 단백질
발현 양상에 미치는 영향 |
| 김선국, 이용만 ·방선웅 ·이애영 ·김재문 ·유택근*·이영호* |
| 충남대학교 의과대학 신경과학교실,해부학교실* |
| The Effect of Benzathine Penicillin-induced Focal Interictal Epileptiform Discharges on the Expression Patterns of c-JUN Protein in the Rat Brain |
| Sun-Kuk Kim, M.D., Yong-Man Lee, M.D., Seon-Woong Bang, M.D., Ae-Young Lee, M.D.,
Jae-Moon Kim, M.D., Taeg-Geun Ryu, M.D.*, Young-Ho Lee, M.D.* |
| Department of Neurology and Anatomy*, College of Medicine, Chungnam National University |
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| Abstract |
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B a c k g r o u n d : Immediate early gene (IEG) is supposed to be linked in the continuous seizure induced long-term changes of specific neurons. We tried to investigate the effects of focal interictal epileptiform discharges on the c-JUN expression in the rat brain which is not clearly understood. M e t h o d s : Epidural electrodes were placed on a male Sprague-Dawley weighing 150~230 g and benzathine penicillin (Pc) was applied cortically. After focal interictal epilep-tiform discharges were successfully identified, EEG was recorded regularly. Cardiac perfusion and extraction of the brain was done at 2, 4, 24 hours and 1 week after the Pc application. Sixteen rats were evenly distributed into 4 groups. Immunocytochemical staining with specific antisera (Santa Cruz) was performed. Results : The epileptiform discharges were induced within an hour after topical Pc applications. At 2 hours after Pc application, c-JUN was moderately expressed in the dentate gyrus (DG) and weakly expressed in the CA3 pyramidal cell, amygdala, pyriform cortex, thala-mus, and neocortex. At 4 hours, c-JUN was minimally expressed in DG and other regions. Whereas, at 24 hours, c-JUN was maximally expressed in the DG and also in the CA3 pyramidal cell, amygdala, pyriform cortex, thalamus, and neo-cortex. One week after Pc application, c-JUN was moderately expressed in the DG and weakly expressed in the CA3 pyramidal cell, amygdala, pyriform cortex, and neocortex. Conclusions : This data showed that even focal interictal epileptic activity can induce IEG encoded c-JUN protein in the specific distant brain regions of a rat until a late period and the expression pattern showed a synchronous and bimodal pattern.
J Korean Neurol Assoc 18(5):617~623, 2000
Key Words : Penicillin G, Benzathine, Focal interictal epileptiform discharge, c-JUN protein |
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