Relationship Between Changes of Biogenic Amines and Free Radicals in the Ischemia/Reperfusion Injury of Rat Brain

J Korean Neurol Assoc > Volume 11(3); 1993 > Article

Journal of the Korean Neurological Association 1993;11(3): 329-340.

허혈/재관류 손상에서 뇌조직 아민 변동과 Free Radical과의 관련성

이경은, 김경환

연세대학교 약리학.

Relationship Between Changes of Biogenic Amines and Free Radicals in the Ischemia/Reperfusion Injury of Rat Brain

Kyung-Eun Lee, M.D., Kyung-Hwan Kim, M.D.

Department of Pharmacology, College of Medicine Yonsei University

Abstract

After the ischemic episode. A new cerebral damage can occur during the restitution of blood flow. Oxygen free radicals. Formed during ischemia/reperfusion. Have been proposed as one of the main causes of reperfusion injury. Altered concentrations of brain biogenic arnines are also reported in ischemia or reperfusion. However, a paucitv in inforrnation and controversy, in the mechanism of the brain darnage during reperfusion still remain. In the present study. Therefore. The ischemia- and/or reperfusion-induced changes in biogenic amines were measured from rat brain regions.. Furthermore. The ability of free radical scavengers (Superoxide dismutase, catalase, allopurinol and deferoxamine) to prevent changes in brain biogenic amines was also investigated. To induce brain ischemia/reperfusion, bilateral common carotid arteries were clamped for 30 minutes and thereafter the.clamps were removed to reperfuse for 15 minutes. The concentrations of biogenic amines and their metabolites (dopamine- 3,4-dihydroxy phenylacetic acid (DOPAC), homovanillic acid (HVA), 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA)) in various brain regions, including frontal cortex, corpus striatum. Hippocampus and thalamus; were measured using the high performance liquid chromatography with electrochemical detector. The results are as follows : 1. In the ischemia group the concentrations of DOPAC and the ratio of DOPAC/dopamine were increased and the ratio of HVA/DOPAC was decreased. Which were evidently only in corpus striatum.