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J Korean Neurol Assoc. 2009;27(1):36-41.
- Molecular Optical Imaging of Cathepsin-B Proteolytic Enzyme Activity
to Reflect Atherosclerosis Pathophysiology and Anti-Atherosclerotic
Therapeutic Effect
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Dong-Eog Kim
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Molecular Imaging and Neurovascular Research (MINER) Laboratory, Departments of Neurology and Pathologya,
Dongguk University International Hospital, Goyang, Korea
- 동맥경화 병태생리 및 치료 효과를 탐지하는
Cathepsin-B 단백분해효소 활성도 광학 분자영상
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김동억, 김정연 김어진a 정상욱
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동국대학교 의과대학 일산병원 신경과, 병리과a
- Abstract
- Background
Protease-sensing molecular optical imaging can potentially identify vulnerable atherosclerotic plaques
before cerebral infarcts occur. However, several translational issues need to be resolved before implementing
human trials and clinical applications of the technique. We investigated whether molecular optical imaging reflects
(1) atherosclerosis pathophysiology and (2) the atherosclerosis-modulating effect of a Western diet or atorvastatin
treatment in ApoE-knockout mice.
Methods
We fed 21 8-week-old ApoE-knockout mice a normal chow diet (n=7), a Western diet (n=7), or a
Western diet with atorvastatin (n=7) for 14 weeks. Blood was collected for measuring cholesterol levels at 24
hours after injecting the cathepsin-B (CatB) probe into the tail vein. Near-infrared fluorescence reflectance imaging
of CatB activity was performed after excising the aorta.
Results
CatB molecular optical imaging precisely reflected the pathophysiologic alteration of atherosclerosis. The
CatB activity signal was significantly stronger in the Western diet group (49.3±26.0 [mean±SD], arbitrary units)
than in the atorvastatin group (25.3±8.7) and the normal chow diet group (20.6±21.1). The effect of the statin on
CatB activity was independent of cholesterol lowering.
Conclusions
CatB-sensing molecular optical imaging represents a valuable tool for evaluating atherosclerosis
pathophysiology and for monitoring the effects of antiatherosclerotic therapeutic interventions. Key Words: Molecular imaging, Atherosclerosis, Optical imaging, Cathepsin, Protease, Statin
Keywords :
- 초록
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