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J Korean Neurol Assoc. 2005;23(6):806-813.
- The Effects of (-)-Epigallocatechin Gallate on Rat Hippocampal Organotypic Slice Cultures Treated with the 1-42 β-amyloid Protein
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Young-Chul Youn
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Department of Neurology, Chung-Ang University College of Medicine, Seoul, Korea
- 쥐 해마 절편배양에서 베타-아밀로이드 1-42분획 독성에 대한 (-)-Epigallocatechin Gallate의 효과
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중앙대학교 의과대학 신경과학교실
- Abstract
- Background
Considerable evidences suggest that the β-amyloid acts as a neurotoxin, and the epigallocatechin-3- gallate (EGCG) has the anti-inflammatory and anti-oxidant properties. The purpose of this study was to investigate whether the EGCG reduces the death of the cultured hippocampal tissues exposed to the β-amyloid 1-42 fragments (Aβ1-42).
Method: We cultured the hippocampus of postnatal 7 days old Sprague-Dawley rat into slices of 450 μm. The tissue slices had been exposed with 100 μM Aβ1-42 at an interval of 3 days since 12 DIV (days in vitro). Following co-treatment of the tissue with 10 μM EGCG and Aβ1-42, we evaluated EGCG effect on Aβ1-42 induced neurotoxicity by measuring the expression of Bcl-2 and NeuN protein and by morphological observation of the hippocampus slice cultures with propidium iodide (PI) and bromodeoxyuridine (BrdU) staining.
Results
The EGCG exerted a significant role in restoration of NeuN protein expression inhibited by Aβ1-42, showed inhibitory effects fluorescence in PI stained tissues, and increased the anti-BrdU stained cell.
Conclusions
10 μM EGCG reduced the Aβ1-42 induced neurotoxicity of the hippocampus slice culture. KeyWords:Alzheimer's disease, Amyloid, Hippocampus, Epigallocatechin gallate
Keywords :
- 초록
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