- J Korean Neurol Assoc. 2002;20(6):660-667.
- "The Selective Cyclooxygenase-2 Inhibitor Rofecoxib Reduces Chronic Cerebral Hypoperfusion-induced Apoptosis in the Rat Hippocampus"
- Young-Bin Choi, M.D
- "Department of Neurology, College of Medicine, The Catholic University of Korea Clinical Medical Research Institute, Holy Family Hospital, The Catholic University of Korea"
- "선택적 Cyclooxygenase-2 억제제인 R o f e c o x I b 가 쥐의 해마에서 만성 저혈류로 유발된 아포프토시스에 미치는 효과"
- 최영빈 , 정성우 김영인 이광수 김범생 이희진 이원선 박상희
- 가톨릭대학교 의과대학 신경과학교실,가톨릭대학교부속 성가병원 임상의학연구실
- Abstract
- "Background : Chronic cerebral hypoperfusion induced by permanent occlusion of bilateral common carotid arteries
(2VO) in rats caused cognitive deficits and neuronal damage. Cyclooxygenase-2 (COX-2) inhibitor was reported to
attenuate both post-ischemic prostaglandin accumulation and neuronal damage. We studied the expression of mRNA of
COX-2 in the hippocampus during hypoperfusion and the effectiveness of selective cyclooxygenase-2 inhibitor, rofe-coxib,
in preventing the neuronal damage of this model. Methods : Bilateral common carotid arteries of the rat were
ligated with silk sutures. The expression of mRNA for COX-1 and COX-2 were detected by the RT-PCR. The first
group of animals (n=6) was treated with rofecoxib (10 mg/kg, I.p.) 7 days after operation and the following 7 days. The
second group of animals (n=6) was treated with diclofenac sodium (9mg/kg, i.p.) and the third group of animals (n=5)
was treated with vehicle (DMSO). TdT-mediated dUTP nick end labeling (TUNEL) technique was performed to esti-mate
delayed cell death. Results : Bilateral carotid artery occlusion (2VO) was shown to induce apoptotic morphology
and DNA strand break in hippocampal neurons from 7 days with a peak at 14, 28 days. mRNA of COX-2 appeared in
the frontal cortex (14, 28 days) and hippocampus (14, 28, 63 days). Treatment with rofecoxib significantly (p<0.05)
attenuated the number of TUNEL-labeled cells in the hippocampus, whereas the cells of the diclofenac treated group
were not protected. Conclusions : We concluded that COX-2 might contribute to cell death of pyramidal cells of the
hippocampus of hypoperfusion and selective COX-2 inhibitor, rofecoxib, could prevent the neuronal damage.Key Words : Hypoperfusion, Apoptosis, Cyclooxygenase, Vascular dementia"
Keywords :