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J Korean Neurol Assoc. 1999;17(5):688-693.
- Protective Effect of Ginsenoside Rb1 and Rg1 Against β
Amyloid(25-35)-Induced Neurotoxicity on B103 cells
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Eun Ah Lee, M.D., In Soo Joo, M.D., Kyoon Huh, M.D., Inhee Mook, M.D.
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Department of Neurology, School of Medicine, Ajou University
- B103 세포에서 베타아밀로이드(25-35)독성에 대한
Ginsenoside Rb1 과 Rg1 의 보호효과
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이은아, 주인수*·허 균 ·묵인희
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아주대학교 의과대학 신경과학교실
- Abstract
- Background
: Ginseng extracts, known to enhance bodily functions including learning and memory, were reported
to have in vitro neuroprotective activity in vitro. Here We demonstrate the possible therapeutic effects of ginsenosides
on the cell culture model of Alzheimer’s Disease (AD). We tested whether Rb1 or Rg1 , major components of ginseng
saphonins, protects neuronal cells from the toxic effect of ß-amyloid (Aß), which is regarded to be the main neurotoxic
substrate in the AD. Methods : B103 cells, rat brain-derived neuronal cells, were cultured and the extent of neuropro-tective
effects of ginsenosides on the cytotoxicity induced by exogenous Aß25-35 was were measured by MTT assay.
Results
: Treatment of Rb1 and Rg1 at various concentrations (l0nM, 50nM, and 1μM, respectively) in B103 cells did
not show any dose-dependent neurotoxic effects. Rg1 (1μM) significantly blocked the neurotoxic effect of Aß2 5 - 3 5
(50μM)(P<0.05). Rb1 at concentration of 1μM also had some neuroprotective effects, but not as effective as Rg1 . These
neuroprotective effects are comparable to the one of estrogen (1.8nM). Conclusions : This experiment suggests the
potential beneficial effects of ginseng in the treatment of AD.
J Kor Neurol Ass 17(5):688~693, 1999
Key Words : Alzheimer’s disease, ß-amyloid toxicity, Saphonin(Rb1 , Rg1 ) Protective effect
Keywords :
- 초록
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