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J Korean Neurol Assoc. 1995;13(4):749-761.
- Effects of Gangliosides on DNA Synthesis in U1242 MG Glioma Cells
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Kwang-Ho Cho, M.D., Min-Choel Lee, M.D
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Department of Neurology, College of Medicine, Wonkwang University, Dept of Patology, College of Medicine, Choonnam University
- Ganglilosides가 U242 MG 신경교세포의 DNA합성에 미치는 영향
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조광호, 이민철
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원광대학교 신경과, 전남대학교 병리학교실
- Abstract
- Exogenously added gangliosides modulate the growth and differentiation of a variety of cells in vitro including human gliomas. GM1, Gdla and GTlb gangliosides inhibit both PDGF-stimulated and serum-stimulated DNA synthesis of Swiss 3T3 cells and U1242 MG cells in a dose responsive manner. The inhibitory effect is counteracted in a dose-responsive fashion by serum, and that ganglioside-induced inhibition is essentially abolished in 10% serum. Because of the potentially important role that gangliosides play in growth regulation of human gliomas, this phenomenon was studied in detail using the human glioma cell line U1242 MG.
Low doses of serum stimulate DNA synthesis of U1242 MG cells which is inhibited in a
dose-responsive fashion by ganglioside GMI. However, serum itself counteracts the
inhibitory effect of ganglioside in a dose-responsive way. On the other hand GM,, Gdla
and GTlb stimulate DNA synthesis in quiescent U1242 MG cells in both sparse and
confluent conditions, indicating that ganglioside-stimulated DNA synthesis is dependent
on the phase of cellular growth rather than celluar density. The growth stimulatory
effect of the three gangliosides is more potent on quiescent, confluent cells than
quiescent, sparse cells. Gangliosides stimulate radiolabeling of and 35% of nuclei with
(3H) thymidine in quiescent, sparse and quiescent, confluent cells respectively.
These results demonstrate that exogenously added gangliosides can have different
effects on progression of human glioma cells, support of the bimodal behavior model of
gangliosides. The effects are mainly related to cell cycle depending on the growth phase
of the cells rather than the cell density.
Keywords :
- 초록
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