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J Korean Neurol Assoc. 1995;13(4):736-748.
- Choline Acetyltransferase Immunohistochemical Studies on Basal ?Nucleus of Meynert and Vestibular Nucleus of Pyrithiamine-Induced? Thiamine Deficient, Rats
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Dae-Il Chang , M.D., Tae-Kyung Baik, M.D.
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Department of Neurology, College of Medicine KyungHee University, Dept of Anatomy, College of Medicine, Hanyang University
- Pyrithiamine으로 유도된 Thiamine결핍 횐쥐의 전뇌 기저핵과 ?전정핵에 대한 면역조직화학적 연구
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장대일, 백태경
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경희대학교 신경과, 한양대학교 해부학교실
- Abstract
- Thiamine deficiency is generally accepted as the primary etiologic factor for the Wernicke encephalopathy in human and for the similar neurologic symptoms in thiamine depleted experimental animals. Although pyrithiariiineinduced thiamine deficiency has been known to produce histopathologic lesions within many nuclei of the rat brain, the pathogenic mechanisms involved have not been clarified. Furthermore, the effect of thiamine deprivation on the nature and anatomic distribution of neurotransmitter changes has not been fully explored.
The present studies were undertaken to investigate - morphological changes of the
basal nucleus of Meynert and vestibular nucleus in thiamine deficient rats induced by
pyrithiamine and thiamine deficient diet. For this purpose immunohistochemical stain for
choline acetyltransferase was performed. Fifty healthy Sprague-Dawley strain rats
weighing about 150 gm, were divided into 10 control group and 40 thiamine deficient
group. Animals in thiamine deficient group were treated with daily intraperitoneal
injection of pyrithiamine( 50 ug/lOOgm of BW/dbLy, Sigma Co.) for 9 days and were
continuously given thiamine deficient diet until to be sacrificed. Thiamine deficient rats
were subdivided into 3 groups according to different stages of neurologic manifestations
; the early group, the beginning stage of anorexia, hypothermia and weight loss without
neurologic manifestations(sacrificed day ; 9th-13th day) the intermediate group, the
developing stage of gait ataxia and hypotonia(sacrificed day ; 17th-19th day) the late
group, the established stage of tremor, convulsion and back arching(sacrificed day ;
23th-26th day).
All animals were anesthetized with sodium pentobarbital(40mg/kg, I.p.) and perfused in
vivo through the ascending aorta with 10% neutral buffered formalin or 4%
paraformaldehyde-0. 1% glutaraldehyde in PBS, and then brains were removed.
Luxol-fast blue and cresyl violet stain was performed according to routine paraffin
method for observing morphologic changes in basal nucleus of Meynert and vestibular
nucleus. In addition immunohistochemical stains in the same regions were performed by
free floating method in cell culture plate. All preparations were observed with a light
microscope.
The results obtained were as follows ;
1. Sequential changes of the neurologic manifestations in thiamine deficient rats were
weight loss, hypothermia and ariorexia on the 9th-10th day, followed by gait ataxia and
hypotonia on the 13th-15th day, and then tremor, convulsion and back arching on the
22th-26th day.
2. Glial proliferation was noted in the basal nucleus of the early group but not in the
vestibular nucleus. Atrophy and pyknosis of neurons in basal nucleus and vestibular
nucleus were shown in the intermediate group and marke neuronal loss and edematous
tissue necrosis were noted in the late group.
3. Choline acetyltransferase immurforeactivity in the basal nucleus and vestibular nucleus
was markedly positive in the early group as well as control group, moderately positive
in the intermediate groupand minimally positive in the late group.
It is suggested that the extent of neuronal damage in thiamine deficient rats is
proportional to the duration of thiamine depletion. And the data presented here may
account for: the regional susceptability and reversibility of certain symptoms in thiamine
deficient rats.
Key Words : Thiamine Deficient Rat, Choline Acetyltransferase, Basal Nucleus of
Meynert, Vestibular Nucleus.
Keywords :
- 초록
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