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J Korean Neurol Assoc. 1991;9(2):157-170.
- Morphological Changes of Somatostation Immunoreactive Neurons in Senile Dementia of Alzheimer Type
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Ki Hyun Cho, M.D., Ph.D., Sei Jong Kim, M.D., Ph.D.
Baik Yoon Kim, M.D., Ph.D.*
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Dep. Of Neurology & Anatomy, Chonnam University*
- 알쯔하이머형 노인성 치매에서의 Somatostatin 면역조직화학반응양성 신경원의 형태학적 변화
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조기현, 김세종,김백윤
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전남대학교 신경과, 해부학.
- Abstract
- Five brains from established cases of Senile Dementia of Alzheimer Type (SDAT) and six branis from cases of no clinical or neuropathological evidences of SDAT were studied immunocytochemically using anti-somatostatin (SST) antibody. Fifty micrometer sections of immersion fixed brains (autopsy delay: 10-16 hours) were cut and then stored in 0.1 M tris-HCI buffer (pH7.4) until the immunocytochemical study. Lmmunostatinings were performed according to Sternberger's peroxidase-antiperoxidase (PAP) technique. The primary antiserum was rabbit antiserum to synthetic somatostatin (Cambridge Research Biochemicals). Diluted to 1: 1.000. The secondary antiserum was goat anti-rabbit IgG (Sternbeger-Meyer) in dilutions of 1: 400 to 500. Controls followed complete staining protocol. But without incubation in the specific antibody. SST-immunoreactive (IR) neurons in aged normal brains were distributed in all layers of cortices. These cells were multipolar, bitufted, or wramidal in shape. The changes of SST-IR neurons were profound in frontal, parietal, and temporal cortices of SDAT brains, but were absent in the Cal of hippocampus. Neuritic plaque-like structure formation was the most common changes seen in the cortices, especially in the temporal cortex. The morphology of altered SST-IR neuronal elements were similar to that of the neuritic plaques demonstrated by the Bielshowsky's silver impregnation method. Apparently intact SST-IR neurons were also found in SDAT brains, thus giving rise to the specu@ation that these neurons may have unusual survival and reorganization
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